How to perform doublet discrimination on a Beckman Coulter FC500 machine?

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Is it necessary to set the current in the edge in infinite elements in Comsol?

Hello, I work in magnetic field physics and consider the magnetic field of a long conductor 3D (I use the current in the edge as a conductor). Due to the large size, I need to use infinite...

03 April 2022 5,559 0 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

To assess the immunogenicity of the SARS-COV-2 RBD protein (CHO-derived) mice and Syrian hamsters were immunized two times (50 μg protein, mixed with aluminum hydroxide adjuvant ) on days 0...

21 February 2021 7,346 2 View

Viability assay for macrophages, infected with bacteria?

Hi, Can anyone please recommend how to measure viability of THP-1 macrophages after infection (stimulation) with Group B Streptococcus? I have tried PrestoBlue cell viability assay but the...

10 February 2021 7,587 5 View

What is your take on the ever increasing number and types of metrics related to scholarly output?

The types of metrics and their number increase and they get more sophisticated (and some might say pervasive). Is it a rather positive or a rather negative development for academia at large?

22 September 2020 7,851 13 View

Any advice on ITC with low affinity compounds (low c-value titrations)?

I am working with several compounds that are expected to have a Kd of ~ 20 uM. They are also poorly soluble in aqueous solutions so it is not possible to titrate high concentration of compound...

30 January 2020 6,214 2 View

Can I retrieve information about the country of permanent residence of a person who is interacting with my posts on Facebook or Instagram?

Is it possible (and how) to retrieve information about the country of permanent residence (not the current location) of a person who is interacting with your social media pages like Facebook or...

09 November 2018 8,998 2 View

Where can I find statistics for outbound tourism of Kuwait?

I need data on outbound tourism of Kuwait.

15 February 2018 2,527 4 View

How we can predict trains traffic regulation by using European Railway Train Management System?

I have been working with research on new predictive model of train traffic by using new railway signaling systems. I would appreciate if anyone could introduce me any work about European Raiway...

08 September 2016 4,467 17 View

Can you suggest econometric techniques that able to measure the impact of several macroeconomic factors on binary variable?

Dear collegues, I am writing a proposal for my new research project and wondering whether any new powerful techniques have been recently introduced to measure the connectedness between factors and...

17 July 2016 975 8 View

Absorption coefficient for gadolinium oxide (Gd2O3)?

Is there any information about the value of absorption coefficient in the plateu region for gadolinium oxide (Gd2O3)?

27 April 2016 3,186 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Can I use CCCP instead of H₂O₂ as a positive control for ROS measurement through flow cytometry?

I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.

20 July 2024 6,354 0 View

I came across oscillations in a pressure profile for a pipeline flow along the axis of a cylinder, how do I prove that these are not numerical err?

In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?

13 July 2024 6,296 5 View