Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View

How to perform cell transfection and Dual Glo assay with low-adhesive (HEK293) cells?

Hello everyone. I need to use Promega Dual Glo assay for HEK293 cells, but I am really afraid of wash out my cells from the 96-well plate, and at the same time, I am afraid to use coating because...

09 September 2020 4,817 7 View

How to reduce auto fluorescence in bone-marrow smears that would be used for FISH and IHC?

We are processing clinical samples of bone marrow smears, and have encountered autofluorescence that makes subsequent FISH and IHC virtually impossible. The problems are: 1. the samples are of...

18 December 2019 4,676 4 View

Antibody detects protein which was ablated in null mice (global knockout). Anyone had similar experience?

Here is a dilemma: there is a line of null mice (global knockout), which are null by genotyping and by mRNA analysis (qPCR). They were generated by replacement of an entire protein-coding region...

16 March 2019 9,722 6 View

How to perform doublet discrimination on a Beckman Coulter FC500 machine?

I work on a Beckman Coulter FC500 machine. Where I can find a (SS or FS) PEAK signal to assign it to AUX for doublet discrimination? There is no such parameter on our list. Thanx!

21 February 2019 9,530 3 View

Does high fidelity taq polymerase get less efficient after expiration date?

24 September 2016 5,468 4 View

Wrong or no fragment insert in directional TOPO cloning?

17 July 2016 10,020 10 View

Why in the analysis of cyanide, ninhydrin dissolved in sodium carbonate?

I was doing research related to the analysis of cyanide with ninhydrin, in some journals mentioned that preparation ninhydrin by dissolving it in sodium carbonate, but I do not understand why. Is...

12 July 2016 8,541 4 View

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15 June 2016 5,924 6 View

Why do I get a wrong length of PCR result but after sequencing it is actually a right target sequence?

Can somebody help me? I am doing a PCR of worm tissue. I used KAPA Taq Extra HotStart ReadyMix. I should be getting a PCR result of 158bp but I always get a 200 bp PCR result and after I sequence...

15 June 2016 7,313 6 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

Can a semi-conductor material have different absorption coefficient?

Does the absorption coefficient of the semiconductor changes with the change in the structure of the semiconductor? If you have a thin film and nanowire of the same semiconductor, will the...

23 February 2021 9,799 4 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View