Hi.

I want to perform a plaque assay using MA104 cell line. After virus absorption, I wash the cells with serum free media and add a 1:1 mixture of 1.2% agarose and Medium( DMEM containing L-Glutamine, Vitamins, Nonessential amino acids, sodium pyruvate, sodium bicarbonate and Pen/Strep). I put the plate in 37C and every 24 hours I check them to avoid gel drying. I check them every 24 hours but after 48 hours, cells die and I can not see any plaques.

Does anyone know how can I keep them alive to continue the test and observe the plaques?