Hi. If I want to do qPCR to certain miRNAs, do I send the company the name of miRNAs or sequence for primer or sequence of miRNA? And do u know a company? .
If you are seeking for SYBR Green-based system you would order from Exiqon, using the link below:
https://www.exiqon.com/mirna-qpcr-search
Along with it, you should order the miRNA cDNA synthesis kit and qPCR kit from the same company.
If you are looking for a TaqMan-based system you should try Lifetech/Thermofisher products. You also need to order the TaqMan® MicroRNA Reverse Transcription Kit and qpCR kit from the same company.
To clarify more, do consider that exiqon system is less accurate but it is less time-consuming. If you need to have very accurate results, I recommend Lifetech/Thermofisher products.
Please take note that Exiqon and Lifetech/Thermofisher procedures are quite different and you cannot mix them to qPCR. So, according to your need, choose one of the companies and order everything from the selected one.
Hi, if you need to analyzed a panel, in a tissue or cell lines, it is better to contact technical support of one or more companies (Lifetech and Qiagen, for example) and ask for help, in any case you can recommend you about products and cost, regarding also your real time machine. From my experience it is better Taqman-based system then Syber with miRNA