Zaynab Saad

10 Questions 33 Answers 0 Followers

Questions related from Zaynab Saad

Is it possible to freeze bacterial cells before comet test ?

Hi everyone it is possible to freeze bacteria cell with saline under -80C for next day ( for e.g) to start comet assay ( determine DNA damge ) !! Or I should use fresh prepared suspension...

18 February 2024 6,351 2 View

FTIR / OPUS software?

Dear researchers We use in lab FTIR with OPUS software all going ok until saving data to get the analyzed data , curves, tables the program results the first training default results !! How i...

28 September 2023 8,478 0 View

Why NaCl buffer cloudy??

good morning i'm preparing RIPA buffer using the following recipe: NaCl 5M Tris-HCL 1M sodium depxycholate 10% SDS 10% only the NaCl was cloudy solution, I've prepared it by weighting: 29.1g...

13 November 2022 6,443 3 View

What can i use instead of HBSS solution?

have a safe and healthy days i want to know what is the best solution to be used instead of HBSS solution in comet assay while using g bone marrow samples thanks

15 December 2020 5,537 1 View

Why different protein activity from multiple codons for the same amino acid???

hii all i'm asking why multiple codons code for the same amino acid but different protein activity ? please attach papers or reviews thank you all zaynab

17 December 2018 9,337 3 View

"kit of pyruvate concentration" for serum samples are good or suitable for cell line suspension??

Have a nice day colleague i'm going to determine pyruvate concentration from cell line suspension (before and after treatment) for different duration times using Elabscience pyruvate assay kit...

21 October 2018 9,734 2 View

Iraq cancer registry 1990-2000??

have a nice day i'm very interested to study the statistic and gradual increase of cancer incidence in iraq specially between 1999-2000 year i search and couldn't find something i can depend just...

09 January 2018 4,379 1 View

How can i prepare skimmed milk?

Hi ... I'm about to do western blotting. ... and i want to block using skimmed milk How can i prepare it ? Or can i use powder one ? Thanks

03 April 2016 7,181 9 View

Any protocol for protein extraction?

Hi ... I've scraped cancer cell line without throughout the media ... all the suspension transfered to a tube and stored at -80 C. How can i extract protein from this one please any ideas or...

30 March 2016 9,709 6 View

Is their any differences between ECL western blotting apparatus and gel band fluorescent detection apparatus?

Hi ... in lab. I have band ge detectionl fluorescent apparatus and I'm undergoing to do western blotting using enhanced chemiluminescence (ECL) My question is ... can i determine the bands on the...

25 January 2016 7,772 2 View