I want to do rat dura mast cell staining but I'm not sure how to mount the whole dura mater on a slide for later washing and staining. Do I need a specific agent to mount/stick the whole dura tissue on slide? Thanks.
I've never seen these kind of peaks before,what reason can cause it,I'm really confused.
13 November 2023 7,297 6 View
Hi all, I set up the crystallization of protein-DNA complex for initial screen and found one crystal cluster appeared in a condition (0.1M HEPES pH7.0, 0.2CaCl2·2H2O, 45% MPD). Afterwards I added...
04 May 2023 5,110 1 View
I set up the crystallization of protein-DNA complex for initial screen and found the condition to optimize (0.1M HEPES pH6.5, 0.2MgCl2, 25% PEG3350). After rounds of optimization, the quality of...
13 April 2023 5,286 0 View
I'm studying the ion-ion plasma thruster, I want to simulate the magnetic filter in it. The thruster I want to study is similar to that designed by St ́ephane Mazouffrea and Denis Renaud which has...
09 April 2023 5,524 0 View
Hi all, Recently I am working on protein purification. 1. affinity column(GST) 2. Add PSP enzyme to remove GST-tag(O/N) 3. GST-tag off 4. gel filtration However, the protein tends to form large...
07 November 2022 9,442 2 View
I am amplifying a target fragment recently. I have done a gradient to determine the annealing temperature, but there are still many specific bands. May I ask you if the amount of template will...
29 June 2021 5,601 2 View
Dear all, I meet a big trouble in ChIP assay. I prepared the cell lysis buffer/shearing buffer containing 1%SDS which helps sonication well. However, 1%SDS damages the epitope of target protein...
24 June 2021 3,763 1 View
I found 3 drugs that can be used as PKA activator: forskolin, 8-Bromo-cAMP, 6-Bnz-cAMP. Forskolin activates cAMP and can activate PKA. The latter two are cAMP analogs that can activate PKA. I'm...
01 June 2021 3,469 1 View
I think both are used mainly for blocking GABAA receptors. Since gabazine is more selelctive for GABAA receptors, is it better to use for studying EPSC? Is it enough to block IPSC by only blocking...
06 March 2021 1,722 6 View
I have got zebrafish knock-out mutants with crisper-cas9 method, I only observed mutant possessed obvious phenotype and did genes qRT-PCR analysis. however, I didn't know how to design next step...
27 June 2020 3,465 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View