Who has analyzed essential oils in hemp and/or hops on HPLC?

I am trying to utilize my equipment as efficient as possible and want to try to run terpenenes (essential oils) on hemp and hops. Has anyone tried this before or does anyone know whom to contact...

17 August 2020 7,267 6 View

Where can I find academic books on sustainability and also graded/extended reading?

I am purchasing books from my designated budget. Academic books on either sustainability, UNESCO 2030 goals, and also graded and extended reading. Any suggestions would be very helpful and...

09 March 2020 4,723 5 View

Can a cross-over design control for regression toward the mean?

04 March 2020 3,302 3 View

Importance of light chain type on isotype control antibodies for flow cytometry?

I am putting together a panel for flow cytometry, for one particular antibody the only available isotype control has a different light chain to the test antibody. My test antibody is BV711-CD25...

10 June 2019 3,466 2 View

Can anyone explain how I choose a surfactant for a water in oil macroemulsion, and how I decide what volumes of each substance to use?

I am trying to create a water in oil macroemulsion with droplet sizes as large as possible (hoping to get between 0.1-1mm). I have no idea what surfactant to use or the volume of each phase to...

31 May 2019 7,376 3 View

I am planning to do a project management study that is related to health. What is the best topic to research on?

Any suggestions i will appreciate

06 April 2019 7,158 8 View

Optimising RT-qPCR for the detection of very low abundace IL-17A transcripts from human PBMC mRNA?

I am performing qPCR to quantify cytokine expression in human peripheral blood mononuclear cells. I have found it very difficult to get consistent results when assaying for IL-17A. Currently I am...

23 January 2019 8,404 1 View

Can DNase treatments to RNA preparations cause damage or loss to the mRNA?

When performing qPCR, I have noticed that cDNA made from RNA preparations treated with Turbo DNase (Invitrogen) prior to reverse transcription produce CT values that are shifted several cycles...

31 October 2018 9,688 5 View

Deaf friends in Dar es Salaam have asked: What research has been done on the signed language(s) used in Moshi and Arusha, Tanzania?

My Deaf friends in Dar es Salaam, Tanzania have told me the the sign language used in Moshi and Arusha is different than the signed languages used in other parts of Tanzania. They asked me to...

11 July 2018 3,549 1 View

Is a cell self-conscious?

10 September 2017 5,686 88 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

What is the molecular size for crystal violet and reactive orange 16?

I am using methyl violet 10B and reactive orange 16 (RO16) dyes for my Ph.D. research. I'm trying to find the molecular size for both of these dyes. It is possible for me to find these dyes'...

01 February 2021 3,333 4 View