Lingdi Nie

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Questions related from Lingdi Nie

Is there a specific PKA activator?

I found 3 drugs that can be used as PKA activator: forskolin, 8-Bromo-cAMP, 6-Bnz-cAMP. Forskolin activates cAMP and can activate PKA. The latter two are cAMP analogs that can activate PKA. I'm...

02 June 2021 3,470 1 View

Is it better to use picrotoxin or gabazine to block IPSC when studying EPSC?

I think both are used mainly for blocking GABAA receptors. Since gabazine is more selelctive for GABAA receptors, is it better to use for studying EPSC? Is it enough to block IPSC by only blocking...

07 March 2021 1,723 6 View

How to prepare stock and working Toluidine Blue?

I want to stain mast cell of whole dura mater with toluidine blue. I purchased toluidine blue powder from Sigma and their instruction to prepare the solution is '0.01g in 10ml methanol'. Shall I...

26 November 2019 4,151 0 View

How to generate a scrambled control of a peptide that won't work?

I have a peptide but couldn't find any existed scramble control for it in papers. I found Mimotopes website which can generate scrambled control peptide by entering my peptide sequence (25 amino...

27 March 2019 7,978 5 View