I have developed a HPLC method for simultaneous determination of Antidiabetic drugs and NSAIDs, I would like to check their drug-drug interactions and their impact In-vitro, would someone guide me on how to proceed?
Hi,
You can use this paper: http://www.ncbi.nlm.nih.gov/pubmed/22737266
or
http://www.ncbi.nlm.nih.gov/pubmed/20738015
Good luck!
Basically it can be done by performing in vitro pharmacokinetic interactions, whereas pharmacodynamic interactions can be properly done only by in vivo methods. The above mentioned methodologies by researchers will give you the detailed procedure..
For studying the interactions between antidipetic drugs and NSAIDs ,we can perform pharmacokinetic interactions in vivo for investigating the effect of NSAID drug on the absorption, distribution, metabolism & excretion of the antidiabetic drug by using animal module like rats and HPLC method for detecting the conc. of antidiabetic drug either in blood or tissues. Furthermore, we can study this drug- drug interactions in experimentally diabetic diseased rats.
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