I'm trying to grow Sulfolobus on 6 well plates for my experiment, the plate has 5ml of solid media and I inoculated 300ul of cell culture on them. I'm incubating the plates in a box with soaked towels at the bottom of the box. I cover the box with an autoclave bag to create a humid environment and then I place it at the bottom shelf of the incubator. but I noticed the media decreased on day 2. and I need 3 days incubation period so I'm concerned by that time the media will evaporate completely. If anyone is experienced with culturing Sulfolobus I would appreciate your help.

Thank you

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