Hi, I'm working on E. coli O157:H1 bacteria isolated from vegetables... how can I do a genetic confirmatory test? PCR but with which specific gene primer?! if there is someone who has worked on it, help me please with kind regards.
Zahraa A. Alshaheeb
To confirm the presence of E. coli O157:H7, you can perform a Polymerase Chain Reaction (PCR) test with specific gene primers. Here are some options:
Please note that these are just a few options, and the best approach may depend on your specific needs and resources.
Detection of E. coli O157:H7 bacteria isolated from vegetables can be done using various methods. Here are some commonly used techniques:
It is important to note that these methods may require specialized equipment and expertise, and the choice of method depends on factors such as sensitivity, specificity, and available resources.
Lyudmil Antonov Aaron Laud Yes, sirs, I ordered several specific primers and now I have got a standard strain of E coli O157... I hope I'll find the gap in the next trial of a PCR reaction
Hussain Ali Rzoqy surely sir, I was cultured and identified the isolates.. but I couldn't characterize between E coli O157 H7 and other species until now.
Many thanks for your helpful discussion and opinions.
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