Could anyone explain (or provide a a reference) how the commonly used ARG4 meiotic recombination rate reporter construct in yeast works?
I found a few articles from around the 90's (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1204546/pdf/ge1284717.pdf for example) but none of them have a comprehensive enough methods section to explain exactly how this works.
Hi,
The paper you cited used ARG4 as a centromere segregation marker, but not for recombination assay.
Here's an example of using ARG4 for measuring recombination frequency.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC45349/
Please look into Table 1 on the 2nd page. There are couple of diploid strains listed.
They have arg4RV and arg4Bg alleles. arg4RV has a frame shift mutation at EcoRV site, and arg4Bg has a frameshift mutation at BglII site. If recombination occurs between these two mutation sites (I know this is a too simplistic example), you will have a ARG4+ segregant.
So, to perform recombination assay at ARG4 locus, you will need to obtain a diploid strain with such two different alleles.
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