I make agarose gels to check my digestions, but it seems that on every gel I run big white 'blobs' occur. We already tried several things like changing agarose supplier, changing preparation method of samples etc.. But it still keeps happening. We use Serva G for DNA staining. We see it always at the bottom of the gels, but in this particular image you can also see it happen in the lanes where the samples are loaded. Please help me get an explanation for this.
Thanks in advance!
Floris Gal
this does not look like a physical gel or sample problem. I think it may be caused by the settings on the imaging equipment you are using. Too much light is coming through where the signal is negative. I would play with the aperture width or the time of exposure settings to lessen the signal intensity
Hey Floris,
I once had a similar problem and I could fix it by changing the staining solution. Maybe you can just ask one of your colleagues for another dye or request a free sample and test it.
Good luck!
Your dye front is showing up as a negative white image. Change the settings on your camera, add the dye to your running buffer or both.
Many thanks for the nice answers, I will investigate this as soon as possible to let you guys know if I got it solved, but I was busy with other business last days. You will hear from me soon!
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