What is the exchange capacity of Dowex 50WX2 resin?

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Why am I unable to attach and send articles?

06 April 2024 3,192 1 View

96-well format microscope slide-like solution?

I'm doing some high-throughput microscopy, currently on glass-bottom 96-well plates. I'm having some issues that I think won't exist on a regular old slide with a cover slip (emphasis on the cover...

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How to prevent debris from a cortex culture?

Hello all Recently, after we stained our cortical neuron culture with cytoplasmatic dye (ViaFluor or CFDA), we noticed that the culture is messy with debris, which are larger and much more...

18 July 2021 3,514 2 View

Solution to nonlinear ODE?

Dear All, Here is question/problem. For y=y(x) and real x, solve the following ODE: y'-y^2+g(x)=0, where g(x) is a given positive function. For instance, g(x)=2e^x/(e^x+1)^2. Any suggestions...

20 March 2020 8,198 20 View

How long to re-equilibrate a system after changing one ion?

Hi All, I have an equilibrated system containing a protein embedded in a lipid bilayer. I intend to study the protein's selectivity for different ions. To do that I produced several copies of the...

02 November 2019 3,158 3 View

Is the 64-channels Quik-Cap Neo Net a good buy?

Dear Researchers, Has any of you had any experience with the Quik-Cap Neo Net EEG cap? I'm about to order a couple, but I don't know anyone who has used it before. Is it simple to use? Easy to...

29 May 2019 2,005 0 View

What are best practices surrounding operating frequency selection in PMSG design?

Dear community! I have been working on a number of lowspeed (

26 November 2018 2,617 0 View

Inducible promoter for a genetic construct in the mitochondria of yeast

I want to construct an S. cerevisiae strain with a mitochondrial genetic construct that expressed under some easily inducible promoter, like the lac operon promoter or something similar. Can...

09 May 2017 3,676 0 View

Why do these white pieces on a gel occur?

I make agarose gels to check my digestions, but it seems that on every gel I run big white 'blobs' occur. We already tried several things like changing agarose supplier, changing preparation...

03 May 2017 1,079 5 View

C2C12 single cell clonal expansion?

I want to generate knockout cell lines (of C2C12) with the crispr/cas method. Then use FACS to get single cells in a 96-wells plate. After cell sorting I want to expand and clone the single cells,...

25 January 2017 5,079 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Jack Silver

According to the link below, the capacity is 6 mEq/wetted mL. As for the type of column, you'll have to pack one, unless I'm not understanding the question.

https://www.fishersci.com/shop/products/amberchrom-formerly-dowex-50wx2-hydrogen-form-50-100-mesh-3/111015220

William Letter

As the method and/or sample change, so does the loading. Pack a column and run a loading study to find the answer.