Hi all,
I read in the literature that when mesure plasma cfDNA with Sytox Green, plasma was diluted with PBS containing 01%BSA. Is this a necessary step? What is the purpose of adding 0.1%BSA. Thanks!
Fang
Hi all I am able to view the electrostatic potential surface using Gaussview to see the calculating result from Gaussian09W of a small molecule. However, the strength is represented by color. I am...
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I coated a stone surface with K-siilcate. Apparently, it does noe seal the surface since the water penetrae the stone easily and the absorption coefficient does not change too much. However, the...
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I want to increase chromatin compaction of cancer cell and to see the function of interested gene in this process. Is there any method to make it?
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I'm trying to extract DNA from 70% ethanol fixed cell suspensions. Firstly, The cells are fixed and stained for cell cycle analysis and collected after flow cytometry. the question is that the...
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does someone knows why the counterparts of virus A infectious clone swapped by same region of virus B cDNA fragments, virus A plasmid changed to low-copy?
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I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
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I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
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If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
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Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
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during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...
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Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
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We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
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I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
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I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
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