Hello.
I am currently trying to make a buffer for protein expression. According to the article, I am to use a buffer with 50 mM NaxPO4, pH 8 (and 0.5 M NaCl).
How do I make the NaxPO4 buffer?
I have previously made phosphate buffers from cold spring harbor protocols https://doi.org/10.1101/pdb.rec8303
(Mixing aliquots of 1 M NaH2PO4 (monobasic) and 1 M Na2HPO4 (dibasic) aliquots to get the desired pH and concentration.... Is this the same buffer, just with another name? Or what to use to make NaxPO4?
Dear Rie Jønsson,
From my experience, you cannot dissolve 1M of these salts in room temperature. I usually use potassium phosphate instead, as it can reach 1M.
Also, pH 8 is slightly above the buffer range of phosphate (about 6.5-7.6); I recommend using TRIS buffer instead.
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