Dear all,
May someone please share a protocol on how to make chemically-competent E.coli DH5-alpha cells? I have a few vials of NEB C2987H highly-competent E.coli DH5a cells and need to make more before the vials run out.
Thanks,
Kind regards,
Maria
A classical protocol in Sambrook's book works well enough: http://cshprotocols.cshlp.org/content/2018/3/pdb.prot101188.full.pdf+html
https://ujemi.microbiology.ubc.ca/sites/default/files/Chang%20et%20al%20JEMI-methods%20Vol%201%20pg%2022-25.pdf
Maria Victorova
Thank you for the suggestions, will try them out.
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