My professor asked me this question and we had a long discussion about it, any Ideas?
I'm thinking about how to separate and purify the protein from the serum, then make monoclonal antibodies against it and then make immunoturbidimetric assay for quantitative measuring or making sandwich ELISA kit, which one of these is the best ? Notice that the protein is in mg/dl and I want to make ready made kit for commercial reason.
You can purify your protein by the following steps:
Salting out: to precipitate your proteins from non-protein components.
Dialysis to separate the salts from your protein
Filtration Gel Electropherosis: to seperate larger protein molecules from smaller ones (according to the size of your protein you can design the size of filtration beads.
Ion Exchange chromatography: by charge of your proteins.
Affinity Chromatography: using tagged substance.
Regarding forming a monoclonal antibody , I think you can give the purified protein chemical and physical properties to the manufacturer and they will design a monoclonal antibody specific for your protein. best of luck.
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