Any fast method to fix bacteria for Atomic Force Microscopy analysis?

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What is the best solvent to disperse zincoxide nanopartices for antibacterial activity?

DMSO or deionized water?

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How we can stabilize and increase the zeta potential of bio-synthesized silver nanoparticles?

my samples agglomerates and show low zeta potential after a week from the synthesis? I use 50 ml of 1mM AgnO3 + 5 ml of bacterial supernatant ( PH adjusted to 9)

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By which mean I can increase the temperature of the AgNO3, bacterial supernatant mixture?

I do not have microwave reactor (100W 2.45 GHz ) in the lab. So, any alternatives please?

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What are the most actual issues in the teaching process of middle east universities?

specially for graduate programs.

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What do you think about MBEC assay as a method for susceptibility testing of biofilms?

Any further techniques you recommend?

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What is the best concentration and volume of PVP to use it as a capping agent in silver nanoparticles biosynthesis?

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Vladimir Dusevich

For bacteria monolayer it takes 20 min (or less) in 2.5% gluteraldehyde. Is it too long?

Omar Farhan Ammar

Thanks All!

Dear Vladimir, most of the protocols take more than 24 hrs. so how you do this with 25 mins please it would be so helpful!

Nazrul Islam

You can used less concentrated glutaraldehyde (i.e., 1.5%). Not sure how long it will take.