Please Which centrifuge I shoud use ultra or normal one ? Which speed and temperature? Should I put some kind of surfactant? I have 100 ML colloidal solution so should I separate it in two 50 ml tubes? And the washing agent do you prefer water or 2mM sodium citrate? Finally how much I should add from the diluent to the pellet?
Ultracentrifuge. Ideally you wash in the mother liquor - the same ionic make up as your starting suspension - in order to minimize the potential for aggregation. Conditions will depend on the size and polydispersity of your starting material - there's no unique recipe. Just centrifuge longer or faster if no obvious separation.