https://www.thermofisher.com/jo/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/media-preparation-from-powder-and-concentrates.html

To make double-strength L15 media (2X L15), you will need to start with the ingredients and proportions specified in the original L15 media recipe and then double the amounts of each ingredient. For example, if the original L15 media recipe calls for 10 g/L of amino acids and 5 g/L of sodium bicarbonate, the 2X L15 media recipe will call for 20 g/L of amino acids and 10 g/L of sodium bicarbonate.

To make 500 ml of 2X L15 media, you will need to weigh out the appropriate amounts of each ingredient and mix them together in a sterile container. It is important to follow good laboratory practices when preparing the media, such as using sterile techniques and reagents, and to store the media properly to ensure its sterility and stability.

If the media powder does not dissolve perfectly, you may need to adjust the pH of the media or use a different solvent in order to improve the solubility of the ingredients. You can also try using a mechanical stirrer or sonication to help dissolve the media powder. If the media still does not dissolve completely, you may need to discard it and start again with a fresh batch.

It is important to note that the specific ingredients and proportions for L15 media may vary depending on the source and the specific goals of the cell culture experiment, and that it may be necessary to adjust the media recipe based on the needs of the cells and the results of the culture.

Hello Choi Myounggwang

You may purchase L15 (2X) media from the link given below. Details are given in the link.

https://diagnocine.com/Product/Leibovitzs-L15-Medium-2X-w-LGlutamine-2X-Liquid-Cell-Culture-Medium-Liquid/65446

Best.