How to make 2.5mM sodium phosphate buffer pH 7.8?

K V V Sagari Kudaligama @K_V_V_Kudaligama

04 April 2015 1 7K Report

I need to prepare 500ml

K V V Sagari Kudaligama

Mix Sodium phosphate monobasic (SPM) and dibasic (SPD) solutions in the proportions

indicated and adjust the final volume to 200 ml with deionized water. Adjust the

final pH using a sensitive pH meter.

ml of SPM 92.0 81.5 73.5 62.5 51.0 39.0 28.0 19.0 13.0 8.5 5.3

ml of SPD 8.0 18.5 26.5 37.5 49.0 61.0 72.0 81.0 87.0 91.5 94.7

pH 5.8 6.2 6.4 6. 6 6.8 7.0 7.2 7.4 7.6 7.8 8.0

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