How can I find history of public goods with reference to world,India and Gujarat?

I Want history details of public goods with reference to world, India and gujarat. Please help if anyone can.

30 October 2020 726 2 View

Agree/disagree Likert scale response options vs. Item specific response options in a questionnaire?

In general, are item specific (IS) response options better than agree/disagree (A/D) Likert scale response option in terms of reliability, validity and quality? I am looking at it from the...

27 October 2020 3,853 8 View

What are the simulation tools for IoT ?

For academic purpose, free and open source simulation tool for internet of things

09 April 2020 7,137 11 View

How to do 3D slicer to CAD or STL file format conversion for FEA analysis?

Hello Sir, So i have a foam based temporary prototype of a component which i want to be 3d printed. I wanted to make a cad model of it via solidworks or catia but it is too complex to model. If I...

28 February 2020 4,193 2 View

Where can I find a full polarimetric SAR dataset with confirmed oil spill?

I am working on detecting and discriminating oil spill on ocean surface using full polarimetric SAR images. Since the freely available SAR data with a confirmed oil spill is very limited, I am...

06 February 2020 9,960 3 View

Tips to find research gap and making a research proposal for PhD Clinical Psychology?

I've done M.Sc in Applied Psychology and I've been applying for PhD Clinical Psychology for more than 1.5 years in a lot of universities in India but my research proposals get rejected because I...

19 January 2020 7,773 10 View

Is it possible to fit the frequency dependent complex conductivity with extended Drude model when imaginary conductivity is negative?

I have frequency dependent complex conductivity data of a strongly correlated material. Since, scattering rate and effective mass of the charge carriers are frequency dependent in correlated...

01 January 2020 5,634 1 View

Could you help in understanding how to interpret given below data ????

20 November 2019 9,478 1 View

How can I calculate daily and hourly solar radiation on a horizontal surface?

I want to calculate daily and hourly solar radiation on the horizontal surface. I have only seen the method for finding extraterrestrial radiation on books and journal papers. can anyone tell me...

18 November 2019 7,663 3 View

Can anyone suggest me what is the role of various radiation extraterrestrial solar radiation/global solar radiation/direct solar radiation?

I have some doubt: 1. What is the correct name to say Global Solar Radiation or Global Irradiation? 2. can we measure the correct value of irradiation at any surface on earth? 3. Can solar...

01 October 2019 963 17 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View