Unable to search ?

23 March 2024 0 2K Report

Why I'm unable to search for anything on the research gate? As soon as I enter the query for research it asks for verification of my network and it's frustrating. Please provide a solution.

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How to solve the problem of unconscious teeth grinding during sleep at night?

One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...

08 August 2024 1,366 5 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,891 4 View

Should Coefficient of Variation (CV) in a biological data necessarily be below 30%?

In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...

27 July 2024 200 0 View

Non-parametric version of the wo-way repeated measures ANOVA?

Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...

24 July 2024 3,282 6 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,071 2 View

How to determine tannin content in fodder pellets?

22 July 2024 8,161 3 View

Need help is coverslipping-Histology samples?

Hello, Our lab has been experiencing sporadic issues with coverslipping. We use the Sakira Tissue Tek Prisma H&E Stainer and a Tissue Tek Glas2 Automated Glass Cover slipper. Periodically,...

12 July 2024 7,014 3 View

I have a doubt in finding the amount of ferromagnetic and antiferromagnetic contribution in a canted system?

Is it possible to differentiate or calculate, the amount of ferromagnetic and antiferromagnetic contribution in a canted system? (Weakly frustrated canted antiferromagnetic system)

25 June 2024 6,210 0 View

TC28a2 cells detaching from 12-well plate after staurosporin treatment?

Hello everyone, I am facing a frustrating issue with the TC28a2 cell line. After treatment with staurosporine at concentrations of 25nM, 50nM, and 100nM, the cells begin detaching during the...

19 April 2024 3,237 1 View

What does a woman look for in a lover?

A woman… wants more intimacy, comprising affection, commitment, and respect for individual identity… Although she knows he wants sex, she is frustrated because this seems to be the only form of...

23 February 2024 5,748 12 View

Research Gate Frustration?

Is anyone else kind of frustrated with the UI and feature-set of ResearchGate?

11 February 2024 5,955 0 View

What should I do when submitting images CYMK but was identified as RGB?

I felt frustrated when I encountered the situation. Upon uploading my figure to HEAMATOLOGICA, I received an "RGB warning." Despite revising the figure in accordance with the suggested procedure,...

14 January 2024 2,024 0 View

Some subjects are not taken into account by SPSS - why?

Hello, I am trying to run a t-test on differences in mental well-being in the last month (average of 14 sentences) between two groups, women I have 200 and men I have only 34 who answered on the...

13 December 2023 8,960 3 View

Is string theory clinically dead?

String theory remains an intellectual feat and helped derive development in physics, inspires new tools and refinement of concepts but never really made it as a complete theory, as is known. For...

06 December 2023 9,415 5 View

Cell culture - why uneven distribution of monolayer?

Hey all my cell culture fellows, i need your help and experience... i am trying to establish a cell line in our lab. i bought a fish cell line and i am now trying to get it startet. But it is...

21 August 2023 1,704 4 View

Why do you make it hard for people to contact each other?

No, you don't have to become Linked In or some other form of social media. But you could be better. Why can't I send messages to people who want my articles? I wish you would let me. I'll...

23 July 2023 4,987 2 View