Hi

Try the RIPA lysis buffer or your could also use ready made protein extraction kits (e.g. Qiagen).

Hope this is helpful.

regards,

Bassem

Thanks Bassem. Have a great day.

Dear Mingcai,  You want to solubilize collagen I, in order to determine its concentration by ELISA kit. My suggestion is selecting ELISA kit, which contain sample preparation. For example Quickzyme has an ELISA kit for collagen I determination and in its insert described solubilization of sample in HCl 12M (1:1 V/V) and heating.

you can see below insert:

http://www.quickzyme.com/wp-content/uploads/2012/07/Manual-QuickZyme-total-collagen-assayjuly-2012.pdf

or ELISA kit from Chondrex:

http://www.chondrex.com/protocol/6021.pdf

These are useful paper for collagen I solubilization:

http://www.jbc.org/content/235/4/989.full.pdf

http://ard.bmj.com/content/23/4/300.full.pdf

http://www.google.com/patents/US3530037

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1185234/pdf/biochemj00688-0071.pdf

http://www.jbc.org/content/260/22/12252.full.pdf

http://www.groupes.polymtl.ca/tissue/Publications/PDFs/MB44.pdf

of course you can use ready to use lysis buffer (RIPA) from many companies too.

some papers used: PBS, pH 7.4, SDS, 0.1%, Na-deoxycholate, 0.25%

best

mehdi

Dear Mehdi, 

Thank you very much for your detailed information, I will check them.

Have a great weekend.

Mingcai