Hello Hello,

I am trying to stain DsRed/GFP lymphocytes in recipient tissue. Tissues are frozen spleen embedded in OCT gel.

I used a primary Rabbit polyclonal to RFP (Anti-RFP antibody ab62341) against RFP to stain the cells.

The problem is it doesn't work.

Tissues were fixed with either acetone or PF 4% and then permeabilised with 0,2% triton.

Pre-blocked with normal mouse serum.

Primary AB concentration 1:100 or 1:200.

Sec AB: Alexa fluor® 568 goat polyclonal to rabbit Ig

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