I am a Concepcion Calvo researcher at the University of Granada. We are studying the microbial diversity in the composting process of sewage sludge. We're doing the isolation of DNA of isolated cultures for subsequent sequencing of the 16S RNA. Also, we are doing DNA extractions from samples of compost for subsequent pyrosequencing.
In our lab we use two techniques: Qubit and nanodrops to know the quality and concentration of DNA isolated. But when we carry out these determinations with the two methods, the results are quite different. I wonder if anyone has experience comparing these two techniques to determine concentration and purity of DNA. Which is the most adequate?
I too found the same differences between Qubit (Invitrogen) and Biospectrometer (Eppendorf). I found the Biospectrometer more consistent.. So I dont use Qubit any longer.
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