RAJAL DEBNATH

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Questions related from RAJAL DEBNATH

Any thoughts on uncultured microbe isolation?

With the involvement of NGS, most of the researchers have moved to desk than to bench. At bench we understand the biological entity with chemicals and pipettes by monitoring them at specific...

28 June 2015 9,286 10 View

Can anyone help with the R code for distance decay relationship?

I am required to do distance decay analysis for my NGS bacterial community along a geographical gradient. What metadata fields are required for the gradient. Will the geographical co-ordinates...

20 May 2015 8,559 1 View

How do I visualize the bacterial genome using the ggbio R package/Circo?

I need help on visualizing a microbial genome sequence obtained from Miseq-paired end reads. I am not sure, when using the tools, how the input file should look. Can anyone help me with generating...

09 November 2014 6,889 4 View

Does anyone have any advice regarding cold active enzyme expression and purification in E.coli?

I intend to express a protein (enzyme) from a bacteria after PCR amplification and transforming into E.coli. The target gene is from a cold adapted bacterium and the enzyme is active at 10...

22 March 2014 2,582 2 View

Is there any correlation between Psychrophiles and GC rich DNA?

I would like to know if Psychrophiles obtained from environments ranging from 0-10 degrees contain more of GC in their genome. I have community DNA (microbial) from cold adapted regions but the...

23 December 2013 8,702 1 View

Can anyone recommend me an universal primer pair for bacterial lipase gene amplification from environmental metagenomic samples?

I am interested in any program for degenerate primer designing other than CODEHOP.

05 December 2013 7,399 1 View

TA cloning failure with pGEM-T easy vector and JM109 supercompetent cells from Promega.

I have been trying to clone 1.5 kb PCR fragments with A-overhangs (High processivity, low fidelity taq polymerase used). But each time I am not able to get the clones. In the first time...

10 April 2013 9,817 9 View

Removal of secondary bands from PCR.

Normal amplification of the particular gene with the same set of primers is routine work for me. But this is how it all got ruined. I am amplifying a set of reaction for sequencing but I am...

18 March 2013 5,295 9 View

PCR conditions in a real time qPCR makes me ponder sometime in real time!!!

RT-qPCR systems have an inbuilt optimized PCR conditions programmed which actually works with most of the primers used. I use an Applied Biosystem 96 well StepONE Plus Platform for comparative...

14 September 2012 7,882 3 View

PCR amplification of 16S rRNA gene failure from total soil DNA

I intend to amplify the 16S rRNA gene from total soil DNA, but am not able to. I isolated the DNA using Epicenter (Soilmaster kit) and also MoBIO (Powersoil kit). The isolated DNA is pure as...

19 June 2012 8,622 22 View