You could use flow cytometry to look at the surface expression of your receptor if there is an antibody made to it that works for flow.

Thanks  but I want to perform western blot analysis. As, I already have checked with flow.

You could have a look at https://www.thermofisher.com/de/de/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/efficient-mammalian-membrane-protein-extraction.html