Swapnil Rohidas Shinde

3 Questions 14 Answers 0 Followers

Questions related from Swapnil Rohidas Shinde

Protein precipitation during dialysis.

Dear all, I am purifying a His tagged protein (theoretical pI 6.06). The elution buffer contains 50 mM NaH2PO4 pH 8, 300 mM NaCl and 300 mM Imidazole. I have to perform CD analysis of the same...

10 August 2017 3,111 6 View

How to prepare a protein sample for circular dichroism (CD) analysis?

I have GST tagged purified recombinant proteins and would like to go for CD analysis. Does someone have the sample preparation protocol for running CD analysis? Also, can I use GST tagged...

06 July 2017 5,382 7 View

I want to study glucose uptake, can anyone suggest non radioactive methods?

I will be using cell lines for my studies.

05 June 2015 686 13 View