Swapnil Rohidas Shinde

4 Questions 15 Answers 0 Followers

Questions related from Swapnil Rohidas Shinde

Protein precipitation during dialysis.

Dear all, I am purifying a His tagged protein (theoretical pI 6.06). The elution buffer contains 50 mM NaH2PO4 pH 8, 300 mM NaCl and 300 mM Imidazole. I have to perform CD analysis of the same...

10 August 2017 3,161 6 View

How to prepare a protein sample for circular dichroism (CD) analysis?

I have GST tagged purified recombinant proteins and would like to go for CD analysis. Does someone have the sample preparation protocol for running CD analysis? Also, can I use GST tagged...

06 July 2017 5,421 7 View

How to isolate plasma membrane fraction of HeLa cells?

I am interested to check the levels of a receptor at plasma membrane in HeLa cells. Can anyone help me with a better protocol to isolate Plasma membrane ? Or is there any other way, any...

20 April 2016 5,480 3 View

I want to study glucose uptake, can anyone suggest non radioactive methods?

I will be using cell lines for my studies.

05 June 2015 726 13 View