How to isolate dental pulp mesenchymal stem cells from deciduous teeth by explant culture method?

More Ankita Das's questions See All

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How combine yolo with Faster R-CNN?

I want a model that is balanced with accuracy or speed, faster rcnn has high accuracy while yolo have fast speed. i am thinking to combine them to get a hybrid model to achieve both speed and accuracy

02 August 2024 3,104 0 View

How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...

14 July 2024 950 2 View

Any suitable Animal models to evaluate Mast cell stabilizers?

I am looking for an in-vivo model to evaluate standardized herbal extract for its potential as a mast cell stabilizer.

14 July 2024 779 0 View

What is the mathematical expressionof theoretical yield strength calculated considering solution strengthening in stainless steel 316L?

In the expression for solid solution strengthening model that has been widely used in the literature use a linear form consisting of strength coefficient, and concentration of elements in wt.%....

11 July 2024 4,476 3 View

How to prepare materials before protein-peptide or protein-protein docking?

Hi all, I am conducting research on screening peptides (20-35 amino acids) with potential for treating dengue fever through protein-peptide docking methods. In the protein database, I found the...

10 July 2024 8,037 1 View

How to insert cholesterol inside DPPC membrane by replacing same number of DPPC molecules in GROMACS?

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30 June 2024 4,160 2 View

About the standard of the FRAP in calculation?

Good morning/day/evening salaam I’d like to ask about the FRAP assay again. I have read some articles which represent the data of frap assay as mg trolox equivalent/L, mg ascorbic acid...

27 June 2024 5,239 0 View

How to synthesize Dichloro(p-cymene)ruthenium(II) dimer in a good yield?

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23 June 2024 9,244 2 View

Analysis of microbial biomass for nutrient cycling?

It will be an honor, if anyone can suggest the standard methodology for analyzing nutrients(N,P,K) in soil microbial biomass

23 June 2024 3,850 4 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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How to isolate lymphocytes from mouse spleen?

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04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

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04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

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04 August 2024 8,894 1 View

Subhash C. Juneja

I have done only on MSCs isolation from Bone marrow. Here is one reprint on dental cells I found. I hope that helps.

Ankita Das

Thank you.

Looks I forgot to include file in last answering. Here is one

Nur Afiqah Mohamad

Our lab use KO-DMEM + serum + GlutaMax + Antibiotics + bFGF. So far it's works.

Thanks Subhash C. Juneja and Nur.