I am working with camel brucellosis that firstly screened using RBT(20Ag: 50 Ab) and then confirmed by cELISA using SAVANOVIR kit. To exclude prozone phenomenon, I tried to make less Antibodies concentration(20 Ab: 50Ag & 10 Ab to 30 Ag ul ) for RBT but still gives negative,
1- Do u have any explanation for this as frequently finding?
22- Do u have different protocol for negative RBT assay to be correlated with positive ELISA result?
3- Do u know better ELISA kit rather than savanovir?
4- Have u ever found a mis-coating problem in the plate wells of SAVANOVIR ELISA kit?
thanks
We apply CFT for RBT confirmation. With which Brucella species was the RBT antigen prepared? We use RBT prepared from B. abortus S99 strain. We do not have any problems with cattle, sheep, goats and buffalos. But we have never studied camel serum before. If the Brucella species that infects the camel is different from the RBT antigen in you, a reaction may not occur. We do not use any ELISA kit for Brucella. I recommend the complement test (CFT).
thanks for answer. Do u recommend any kit or protocol for CFT, For rapid serological tests, we always face problems of nonspecificity or low sensitivity
Hello,
I recommend the CFT too. It is good and has international standardization. I would also add the SAT (serum agglutination test). In addition, I would test the camels after a month to see if these antibodies' levels are increasing, decreasing, or maybe they have already disappeared entirely. During brucellosis, the antibodies mostly persist, and in most cases, their levels increase. It would also be helpful to see if these antibodies are IgG or IgM. A rivanol or 2-mercaptoethanol test is very helpful for this purpose.
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