I used by mistake rnase a instead of rnase T1 and now I have to clean all my instruments.. What is the best possible way to do so? I use them for ISH
Other than Sparids
27 June 2024 1,809 0 View
1. Vachellia drepanolobium 2. Vachellia horrida 3. Vachellia karroo 4. Vachellia nilotica 5. Vachellia oerfota 6. Senegalia polyacantha ? [email protected] +2348061166247 Thank you.
05 June 2024 4,858 1 View
Hello, I'm writing Python scripts for Abaqus and I'm facing a problem. I need to change the coordinate system in a .odb file before extracting data but I'm stuck. I can create my coordinate system...
30 May 2024 7,431 3 View
Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...
12 May 2024 8,107 2 View
Dear community, I want to perform an analysis of the risilin localization within the legs of myriapods and would like to visualize the localization of the resilin components in different...
07 May 2024 9,160 3 View
I would like to use the "create.matrix" function (R package "fossil") to create a matrix of taxa, localities, time and abundance. In the function, time is specified by the arguments "time.col" (=...
28 April 2024 3,545 2 View
I am trying to do a golden gate cloning but the ligation-digestion seems to be failing
01 April 2024 6,416 2 View
Hello, What would be the best procedure right after extracting brain samples from mice, if we plan to analyze the samples later ? Would it be best to just directly flash freeze the brain...
28 February 2024 7,384 1 View
Right now I'm working on a senior project in combinatorial game theory - I've read Knuth's book "Surreal Numbers" and I've started reading "Winning Ways for your Mathematical Plays" by Conway,...
20 February 2024 1,254 1 View
What is the Current situation with poverty and inequality in Bangladesh? What are the potential sources/causes of poverty and inequality in Bangladesh? What are the consequences poverty and...
17 February 2024 4,166 0 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View