How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

Safety of the SARS-Cov-2 mRNA vaccine?

Dear colleagues I have the following questions concerning the safety of mRNA vaccines targeting the spike protein of SARS-Cov2: 1) The vaccine elicits antibodies which block the peptide domain...

04 February 2021 721 6 View

Why dose plasma AngII concentration decrease in AngII infusion mice detected by AngII ELISA kit?

I detect the plasma AngII concentration by AngII ELISA kit (ENZO), the results show that plasma AngII decrease in AngII infusion mice. I check with the kit and find that the kit could recognize...

31 December 2020 8,838 3 View

Can RNApolymerase bind to RNA?

Given that the core enzyme tends to DNA in the cell, because of the electrostatic force. Why is there no such tendency between RNApolymerase 2 and RNA?

14 December 2020 2,708 5 View

Can anyone recommend good primary and secondary resources on women perpetrators of the Holocaust please?

I am starting my dissertation for an MA in Cultural History, Memory and Identity. I am interested in examining women perpetrators in the Third Reich; from denunciation to frontline killing. I am...

09 October 2020 4,285 4 View

What is the step up(touch up) PCR? Could you explain?

Can anyone explain the step up PCR? The following is the paragraph, I could not understand the PCR condition for step up PCR [For the DNA-dependent RNA polymerase II second largest subunit...

19 July 2020 5,686 5 View

Can Dynasore inhibit Caveolae-mediated endocytosis?

HI, I'm studying endocytosis pathway of certain particles, using Dynasore and MbCD endocytosis inhibitors. My question is, that whether dynasore could inhibit both clathrin-ME and...

08 May 2020 9,372 1 View

Are proper guidelines being followed for conducting clinical trials related to covid-19 or some compromises are being made on name of global pandemic?

Need for guidelines like GCP, ethical guidelines arose whn it was observed that the rights, health and well-being of participants was taken for granted specially during disasters like second...

30 April 2020 3,952 6 View

Link between ACE-I or ARBs in case of COVID-19?

Is there any scientific evidence linking angiotensin-converting enzyme (ACE-I) or angiotensin II receptor blockers (ARBs) to the risk of COVID-19 contracting or severity?

27 April 2020 3,636 3 View

Could areas of high Fluoride ingestion be more susceptible to Coronavirus outbreaks?

The US Government Comparative Toxicogenomics database shows that Fluoride can inhibit Human immunity to viruses and pneumonia. Angiotensin I-Converting Enzyme (ACE), 2'-5'-Oligoadenylate...

03 February 2020 6,459 4 View