I am looking for an alternative product/protocol to Promega's discontinued HeLaScribe Nuclear Extract kit (E3110) for in vitro transcription. What I need is RNA pol II & associated transcription factors that would recognize a CMV promoter.
Zymogen makes kits for gDNA extraction.
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Why Spine gourd fruit become red while it is tender ? No attack of insect pest is recorded.
11 June 2024 8,771 0 View
One of the major objectives of Natural Farming is self-reliant farmer and promotion of traditional varieties so that the farmers can use their own seeds. But it is observed that most of the...
11 June 2024 5,696 0 View
I am working on Protein-DNA/RNA docking and using HADDOCK 2.4 server, but I couldn't understand the relation between HADDOCK score and binding enthalpy (if there is any). I want to find the...
09 June 2024 3,939 4 View
I have estimated various river morphometric parameters using satellite data and DEM for a river basin. Changes in stream parameters such as sinuosity and channel shifts over a period of time was...
08 May 2024 653 0 View
#rhf/3-21g pop=nboread RHF/3-21G for formamide (H2NCHO) 0 1 H -1.908544 0.420906 0.000111 H -1.188060 -1.161135 0.000063 N -1.084526 ...
05 May 2024 4,500 2 View
Hi, I am new to gaussian and was wondering how to use KMLYP and BHHLYP in the gaussian input file
28 April 2024 6,606 1 View
I want to analyze alcoholic beverages using FTIR for SO2 content and other metabolites. Please guide me
15 April 2024 8,620 0 View
Options are a)40 b)80 c)20 d)60 Explain the answer.
09 April 2024 163 1 View
Want to study GHG emission from small ruminants in small chamber (low cost), How to make chambers airproof, if anyone having experience kindly share your experience with photo please. Thanking...
09 February 2024 5,288 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View