How to induce apoptosis and necrosis in PC3 cell lines without treating with any chemotherapeutic drug?

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Rahul Raghavan .TV

Serum starvation may induce apoptosis in PC-3 cell lines.

Heshu Sulaiman Rahman

Please try serum starvation and over confluency. TQ

Konstantin Golovine

Try irradiation.

Gajendra Shrestha

Thanks all. Could you please suggest how long should I serum starve or irradiate?

Martyn Alun Sharpe

Place them in the bottom of the fridge for three hours and then put them back in the incubator.

After two hours fix them.

serum starvation : Grow fully grown PC-3 cells in media with no serum for 24 hours or more. (need trial and error )