Rahul Raghavan .TV

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I am trying to clone a lentiviral vector (size 12.5kb) into stbl3 cells but I am not getting positive colonies?

What are the parameters to be taken care to transform large size vectors into STBL3 competent cells. I cant work in DH5alpha cells as it is reported to cause recombination to lentiviral vectors. I...

23 July 2015 8,902 16 View

Cells are fixed using 70% ethanol. What will be the chemical effect of 70% ethanol on the membrane of cells?

(modified question after reading comments till 19/9/2013) Thank you all for very informative comments. I read alcohol is used to esterify free carboxylic acid (non-nutral) hope no such reaction...

28 August 2013 5,465 15 View