We are interested to develop a method for predicting lipid binding proteins, I will highly appreciate if some one write "How to create dataset from PDB or any other database" . Please send link if you know any existing dataset.
Dear All I need help in troubleshooting mossy fiber recordings. All my PhD and Post-doc experience so far was in SC-CA1 recordings (Both field and whole-cell recordings). For a new project, I am...
12 February 2024 8,873 3 View
Lumateperone (ITI-007, CAPLYTA™) is an antipsychotic agent, discovered and developed by Intra-Cellular Therapies, Inc. (ITCI) and approved for treatment of schizophrenia in adults in December...
24 October 2023 4,671 0 View
In recent years, number of vaccine have been approved to fight against Covid-19, list of approved is available at FDA site. We are looking for sequence of these vaccine (RNA sequence in case of...
08 July 2023 458 4 View
10gm of plant/ fruit powder was added to 100ml of solvent and subsequently incubated in rotary shaker followed by centrifugation and rotary evaporator evaporation. Afterwards 40ml residue/...
09 January 2023 1,289 0 View
Hi all, I have used the below code for analyzing hbonds in my protein, but I am facing the error below. Please provide solution for this error? python3 readHBmap.py -hbm hbmap.xpm -hbn rg.ndx -f...
26 January 2022 7,142 5 View
I am not sure if any one study on this topic. It has been observed in certain cases that success-full researchers have poor academic record. These are exceptions, I am interested to know any...
30 October 2020 5,312 23 View
I am looking for cell penetrating small molecules that can penetrate membrane of cell. I wants to design molecule that can deliver drugs inside a cell. I know number of databases/methods are...
06 June 2020 4,105 7 View
I am looking for non-protein allergens, please write name of database or repository or link maintain list of allergens.
06 June 2020 1,881 4 View
Despite tremendous advancement in the field of vaccine design or immunoinformatics, most of the vaccines under trail against novel coronavirus are based on traditional approach. In most of the...
28 April 2020 1,291 29 View
Dear all, Im simulating Friction stir welding using Abaqus with CEL technique. It include 2 stages - 1) Friction stir weldingstage, 2)Cooling stage (temprature releasing) In 1st stage i'm using...
11 March 2020 7,469 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View