We are interested to develop a method for predicting lipid binding proteins, I will highly appreciate if some one write "How to create dataset from PDB or any other database" . Please send link if you know any existing dataset.
I am not sure if any one study on this topic. It has been observed in certain cases that success-full researchers have poor academic record. These are exceptions, I am interested to know any...
30 October 2020 5,098 23 View
I am looking for cell penetrating small molecules that can penetrate membrane of cell. I wants to design molecule that can deliver drugs inside a cell. I know number of databases/methods are...
06 June 2020 3,776 7 View
I am looking for non-protein allergens, please write name of database or repository or link maintain list of allergens.
06 June 2020 1,673 4 View
How to relate activity and concentration scale of H+
29 June 2018 4,897 2 View
Hi all, I am designing a student profiling system (in university level technical education) in which student competencies are mapped and used for further intervention. I want to test achievement...
31 May 2017 1,552 11 View
Are the nanoparticles functionalised or used directly? If functionalised, please brief me through the process of functionalisation.
25 December 2014 566 2 View
I am trying to synchronize Raji cells which are suspended cells. But after 24 h of serum starvation they attached to the flask. Does anyone know why they are doing so and how can that be prevented?
12 December 2014 1,712 3 View
As all of us know that Ebola virus is one of the major challenge for researchers community. I hope number of researchers are already working on this dread virus. As well as number of researchers...
14 August 2014 9,563 7 View
We have dataset containing large numbers of proteins belonging to PDB, SwissProt, TargetDB and unknown sources. We have no idea about their nucleotide sequences, but we are interested in...
14 April 2014 403 16 View
I want to make a rotating reactor in which a mixture of dolomite and lump ore are injected from feed end and air injected from discharge end counter currently with discharge end.
25 September 2013 5,769 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...
01 March 2021 3,703 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...
24 February 2021 1,384 3 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
23 February 2021 8,155 5 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...
16 February 2021 2,377 7 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View