Marcel Vlig

3 Questions 3 Answers 0 Followers

Questions related from Marcel Vlig

How to increase yield of RNA of low bacterial concentration?

Hi there, I am looking for a protocol to isolate RNA using low total CFU of bacteria (S. Aureus). We already have a good isolation protocol for mammalian cells (cell culture and tissue), but now...

24 January 2023 7,057 3 View

Centrifuge requirements using Ficoll/Percoll for monocyte isolation?

Hi, I would like to know if some of you had experience with the difference between fixed angled rotors versus swing-out buckets for the centrifuge steps in Ficoll/Percoll protocols for isolation...

28 October 2019 7,531 6 View

What is the best procedure for fixation of cells when antibodies for surface and intracellular markers are need to be analysed?

We are working on a protocol for the isolation and characterization of macrophages from human tissues (skin). We use the following antibodies, CD40, CD80, CD163 and CD207 (all surface markers) to...

24 June 2019 6,458 4 View