How to increase the viability of single cells derived from lymph node and spleen?

02 August 2018 1 9K Report

I am preparing single cell suspension from mouse lymph node and spleen by mechanical dissociation but the viability is is not that good ~80-70%. Are there any tricks to increase the viability?

Amal Ali

Hi Gulab,

I'm keeping them in PBS+ 2% FBS+ 2mM EDTA

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