4 Questions 3 Answers 0 Followers
Questions related from Amal Ali
Has anyone used RNAscope to detect bacterial colonization of mice/human tissue? If yes, Have you used ACD recommended protocol or have you adjusted the protocol to get a decent signal?
11 December 2019 2,778 0 View
I have tried to enrich for T-regulatory cells from Lymph node and spleen of B6 mice and could not get more than 40-50% purity for CD4+ population. Have you tried the kit with >80% purity. What...
02 October 2018 7,235 4 View
I am preparing single cell suspension from mouse lymph node and spleen by mechanical dissociation but the viability is is not that good ~80-70%. Are there any tricks to increase the viability?
02 August 2018 9,149 1 View
I aim to prime T cells with irradiated APC. So how long can I co-culture the irradiated APC without losing their viability?
15 May 2018 2,789 5 View
