How to increase the storey drift of a building beyond permissible limits?

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Which source is authentic regarding systematic classification? Printed standard taxonomic keys or Online databases?

While dealing with systematic classification we are following the standard taxonomic keys. A taxonomic key or guide gives us information about the identification, nomenclature, classification, and...

19 April 2024 9,054 0 View

Good Morning, i am going to perform cell wall loosening enzyme assays?

Good Morning, i am going to perform cell wall loosening enzyme assays in wheat anyone who has done and want to share his/her protocol will be highly appreciated. My focus is on XET and expansins....

04 February 2024 9,963 0 View

Does anyone have a protocol available for the isolation/selection of CRISPER/ KO CD34 cells from wild-types ?

I performed an experiment involving the knockout (KO) of XYZ gene in CD34+ cells, followed by their differentiation into MKs. I am now seeking a reliable protocol for sorting the KO cells from the...

30 January 2024 8,319 0 View

I annotated few draft genomes using AntiSMASH. I can see results with the individual html files. I'm wondering how to visualize results for a batch?

I have about 40 draft genomes binned from metagenomes. I want to see their secondary metabolite production potentials. I used AntiSMASH in the command line. From the output files, I can see the...

29 January 2024 9,711 0 View

Is it possible to estimate the fluid flow behavior in the weld pool by analyzing the temperature distribution in the fusion zone?

27 December 2023 1,654 3 View

For the acoustic design of an enclosure, is it enough if the natural frequencies of the enclosure do not match the frequency of the sound generated?

For the acoustic design of an enclosure, is it enough if the natural frequencies of the enclosure do not match the frequency of the sound generated? Such an approach to vibration would be fine. Is...

30 October 2023 7,314 3 View

How to lower the molecular weight of iron dextran(iron isomaltoside)?

Procedure of making my samples. i Weighed 1 g of dextran with a molecular weight of 1000Da, add 4 mL of water, and dissolve in a 550C water bath. After dissolution of dextran properly i added...

11 October 2023 7,459 0 View

Is there any validated scales for measuring construal level of environmental threats?

Within construal level theory literature, the construal level manipulation check is often takes place with scales such as behavioral identification form (BIF). This scale measure abstract vs....

05 October 2023 5,539 0 View

What are the easiest ways to measure ROS in a specific brain area in free floating brain sections? How stable are the ROS products after perfusion?

I would like to measure ROS by measuring a good indicator of it such as lipid or protein based peroxidation markers etc. Do i need to perfuse he animal with PFA or saline, does PFA fixation affect...

04 September 2023 2,129 0 View

DLS of my xerogel samples show very high PDI value (>1)& very large standard deviation.Can I interpret any result out of this ? Or I need to repeat?

Greetings, I have perfomed DLS of my xerogels but the pdi >1 , between 1.2-2 and standard deviation is very large (300-700) . Can I interpret my result with this data or I should repeat.

08 August 2023 6,734 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View