I want to detect a protein that is present in full length or truncated. I mostly want to detect the truncated version, about 14 AA from the amino-terminus but the antigenicity of the peptide is very poor. Is there a way to modify the sequence to increase its antigenicity?
Thanks!
Could you try fusion to something like the FH8 tag?
Several good papers from Costa et al. in Portugal, well worth reading and seems to have decent performance in a variety of systems.
Best of luck, Robin
i.e. - the paper where they use the tag to improve immunogenicity :
http://dx.doi.org/10.4161/bioe.26003
Conjugating the peptide to a highly antigenic carrier protein such as keyhole limpet hemocyanin is a method that is used to increase the antigenicity of a peptide.
If your peptide/protein sequence is homologous to the sequence of the protein in the host animal, immunogenicity is usually negligible.
You can change the AAs that are not critical for binding by AAs with greater Antigenicity Index.
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