Natascha Techen

5 Questions 11 Answers 0 Followers

Questions related from Natascha Techen

Agilent Bioanalyzer 2100, orange G DNA loading dye, PCR?

The PCR products I want to analyze on the 2100 Bioanalyzer contains DNA loading dye for agarose gel analysis (sucrose and orange G). I added it before loading on an agarose gel. Now I want to...

24 August 2023 4,224 0 View

Efficient way of batch cloning of PCR products?

Hi, I need to clone 30-40 PCR products. I tried direct sequencing of the products but found out that they are mixed and result in overlapping peaks if directly sequenced. I want to clone these...

06 November 2020 10,061 3 View

How to improve antigene amino acid sequence to make good antibodies?

I want to detect a protein that is present in full length or truncated. I mostly want to detect the truncated version, about 14 AA from the amino-terminus but the antigenicity of the peptide is...

14 September 2017 3,616 4 View

Is there a software that can suggest which primer combination should go in a multiplex PCR?

I have designed 30 oligo pairs and found out that some of them can build dimers. I would like to use a software to help me suggest which of the 30 oligos can be in one pool and which should go...

01 July 2015 5,588 9 View

What could this double band in the colony PCR be?

I performed PCR on genomic DNA with gene region specific primers that have a M13 adapter, which resulted in two PCR products. I treated the PCR products with ExoSap (primer removal) and cloned...

01 January 1970 8,805 5 View