Efficient way of batch cloning of PCR products?

Hi, I need to clone 30-40 PCR products. I tried direct sequencing of the products but found out that they are mixed and result in overlapping peaks if directly sequenced. I want to clone these...

05 November 2020 9,898 3 View

Hand Scoring of MIQ?

I used the Minnesota Importance Questionnaire (Ranked version) with a psychotherapy client and then noticed in the manual that the answer sheet is normally computer scored. 1. Does anybody have...

06 August 2020 840 2 View

Are you aware of research on how to best deal with trauma after school shootings?

I am looking for information to help youth with trauma after school shootings. Primarily, I am seeking information on how to help youth at different schools who were not directly involved but,...

22 May 2018 3,564 3 View

Thermal conductivity - theory/background literature?

14 September 2016 8,936 5 View

Is there a protocol for a second immunostaining of organotypic hippocampal slices?

Hello everyone, I am working with organotypic hippocampal slices and months ago I performed immuostainings, to visualize neurons and cell death etc. Now, I want to stain the same slices again with...

26 January 2016 6,807 5 View

Is there a validated scale which investigates parents supportive behavior in their children's mathematics education?

I am working on parental beliefs about mathematics and it teaching and learning and want to investigate, in which ways parents support their children with their mathematics education. Therein I am...

19 October 2014 225 16 View

How can we detect LC-3 II band with western blots?

I also have a problem with detecting the LC3II band on my western blot. The lC3I is not well separated from my LC3II band or the LC3II band is a smear. I use 4-20%Tris/Glycine gels from Biorad....

16 November 2013 5,057 6 View

What could this double band in the colony PCR be?

I performed PCR on genomic DNA with gene region specific primers that have a M13 adapter, which resulted in two PCR products. I treated the PCR products with ExoSap (primer removal) and cloned...

01 January 1970 8,541 5 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Luciferase assay without luminometer?

Hi everyone. I intend to carry out a luciferase assay for my research but have no access to a luminometer. Is there an alternative assay that I can use for my research or can anyone suggest a way...

05 December 2020 9,749 2 View

What is the maximum passage number of SKOV-3 and SKOV-3-Luc cells?

Hello, for my experiment I'm working with SKOV-3-Luc cells, which are SKOV-3 cells stably/continuously expressing the firefly luciferase enzyme. These cells are obtained by the transfection of...

25 November 2020 3,219 3 View

Luciferase assay for gene-miRNA interaction: which vectors should be used?

23 November 2020 2,813 2 View

Does anyone have experience in TOPFLASH assay in HEK293T cells?

I did lipofectamine transfection in 12-well plates with 0.5M of cells and 0.8ug of TOPFLASH plasmid in each well. After 24h, I changed to media to serum-free media supplemented with 10uM...

20 October 2020 3,584 2 View

Luciferase assay for miRNA-gene: How to construct the mutated prime?

I have experimental evidence (qRT-PCR and WB) to suggest a miRNA downregulates a gene. To test if the regulation is direct I want to perform a luciferase reporter assay. So I designed a primer...

15 October 2020 7,641 4 View

Paper authorship for students?

I had a few students help me with a simple but time-consuming task. The data they helped with will be used in a scientific paper. The students are part of the Student Research Program (SRP) at my...

11 October 2020 4,514 12 View

Can we detect neuroprotection, ischemic tolerance, ischemic pre conditioning using fmri ?

I want to know whether there are any non invasive techniques to detect neuroprotection in humans

02 October 2020 5,896 3 View