How to image endogenous RNA in living cells using Mango aptamer?

29 January 2020 0 5K Report

Hi, everyone!

I have constructed lentiviral/retroviral vectors encoding Mango aptamer tagged gene of interest. However, I couldn’t observe the fluorescent signals of TO1-3PEG.

I need an information for the protocols of live imaging of endogenous RNA such as lentivirus-introducing genes.

Is Mango aptamer only useful for in vitro synthesized RNA?

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