Does anyone use TrypLE Express for primary microglia isolation and culture?

14 July 2021 1 786 Report

I'm planning to culture primary microglia from newborn mice. I'm not sure which method of microglia isolation. Does anyone use TrypLE Express for primary microglia isolation and culture?

Plsease tell me the yield of using TrypLE Express over mild-tripsin.

Ji-Won Lee

I also cultured primary mouse microglia from newborn mice.

I used 0.05% trypsin/EDTA solution.

It really works well.

The purpose of the cell dissociation enzyme for primary microglia culture is brain dissociation. If you are using such mild-trypsin solution, it may cause less tissue dissociation.

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