In my experiment I have found a/some metabolite(s) from a strain of Pseudomonas aeruginosa, which could inhibit the growth of Staphylococcus epidermidis. I have confirmed that this metabolite is small molecular compound. After checking the metabolite database of P. aeruginosa, I think it may be phenazine or pyrrole derivatives. But it is really difficult to identify this metabolite(s) further.
So I want to ask: are there any methods to screen the chemical nature of this metabolite(s)?
I appreciate your help and your patience. Thank you!
Thank you Khanam.
Actually I do not know the chemical nature of my target compound. It is just my guess. Can I do some experiment first to narrow down the range of my target compound?
Thank you very much!
Hello Wang, you already know what to do. Perform some biochemical tests to know the probable chemical nature of your metabolite (protein, carbohydrate, lipids). Next, go for the "Eye of the Organic Chemist" It is called Thin Layer Chromatography (analytical and preparative). You can move from here to HPLC, FTIR spectrometry and GC-MS. By the time you arrive here, you will know exactly what you have. It's a promise.
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