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Questions related from Wang Haichen
I am using 1% CTAB for extracellular DNA extraction, followed by isopropanol precipitation, phenol chloroform extraction, ethanol precipitation and wash. However, the final eDNA yield was only...
26 June 2025 9,561 3 View
I am wondering how to extract the pan-genome families from the results of OrthoFinder2. In ref (10.1128/spectrum.02072-21), they said "The OrthoFinder output files (Orthogroup_ Sequences folder)...
17 December 2022 7,522 0 View
Hi, I have to do a bacterial conjugation experiment for the first time. My donor strains are fosfomycin resistant isolates of klebsiella pneumoniae and the acceptor is E. coli J53. However I have...
21 August 2019 2,475 4 View
In my experiment I have found a/some metabolite(s) from a strain of Pseudomonas aeruginosa, which could inhibit the growth of Staphylococcus epidermidis. I have confirmed that this metabolite is...
11 December 2018 7,344 2 View
We all know that the Dubois method is used to measure polysaccharides. I am wondering that if Dubois method is negative, could I consider there is no polysaccharides and even monosaccharide in...
27 March 2018 7,057 5 View
Now I am trying to purify polysaccharide from one strain of Pseudomonas aeruginosa. The medium is M63 with 0.4% arginine. I used 60% cold ethanol (final concentration) to purify the polysaccharide...
31 August 2017 1,692 4 View
Sorry I have a very simple and basic question. Now I am screening the ability for S. aureus and S. epidermidis to form biofilm in 96-well plates. The media is TSB with 0.25% glucose. After 24h...
13 December 2016 4,980 8 View
In my experiment I study biofilm formed by Pseudomonas aeruginosa. And the strains include Pseudomonas aeruginosa PAO1 and several clinical strains and their ability to form biofilm remains...
03 July 2016 8,972 6 View
