What is the best way to create a Vector file for any figure?

Some journals ask for a vector file for the figures/diagrams submitted for the article. What is the best way to create a vector file from your Microsoft Excel chart?

01 February 2021 6,344 2 View

Is it possible that the strain hardening exponent(n) for Aluminium 1060 alloy is greater then 0.3?

I have got the strain hardening exponent value 0.5637 according to ASTM E646 standard.

29 April 2019 3,148 3 View

How to know surface coverage of thin film on the electrode?

After preparation of thin film on the electrode, how one can know its surface coverage

29 March 2018 4,964 1 View

Which guidelines do we follow in selecting sample size?

Scientific survey need practically large sample size. Is there any concrete guidelines? Pl throw some light on it

26 January 2018 4,044 13 View

What is rotated component Matrix in Stastical analysis?

Whenever analysis of data is made through SPSS, we get rotated component Matrix. How do we understand the value that we get and what could be its significance.

31 December 2017 6,560 10 View

Is EMOTIVXAVIER the software to Get Green signals in the software for the detection of sensors of EPOC sets?

The sensors of EPOC Headset are not getting detected but somehow the Cognitiv Suite is showing response. Is it noise which I presently feel to be the response. Please guide me how I can get the...

09 February 2015 1,479 1 View

How to decide a dose of drug to be incorporated, while studying Caco-2 permeability study?

I am going to perform permeation study of some drugs. I got the whole process for the same, but I am not getting how to decide the dose/ amount of drug to be incorporated in the donor compartment.

16 November 2014 9,313 2 View

How one can find the location of drug in polymeric micelle (whether in the core or pallisade layer)?

Can we assume from FTIR that our drug is encapsulated in micelle or not or we must have to go for 2-D NMR?

17 August 2014 4,553 15 View

Why colleagues don't cooperate? Why do they hesitate to accept good work of colleagues?

I have observed that most of the people are always happy to create hurdles and happy to see others distressed. Have you also experienced such situation?

01 January 1970 4,389 37 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View